HPLC as a classical tool for screening of β-Thalassemia and Hemoglobinopathies in Kanpur

Background: Hemoglobinopathies and betathalassemia is one of the most common autosomal disorders worldwide different molecular mechanisms, most of which are base substitution or small deletions or insertions of one or two nucleotides in the globin genes. It has been found that hemoglobinopathies and β-thalassemia mutations are relatively populations specific; each ethnic group has its own set of common mutations. Aim: High Performance liquid chromatography (HPLC) forms an important tool for accurate and speedy diagnosis of various haemoglobin disorders. About 50 cases have been studied for various haemoglobin variants from Kanpur and adjoining area. Materials and Methods: The study was performed Agilent 1220 Infinity LC (Agilent Technologies) a High Performance Liquid Chromatography (HPLC) using beta thalassemia program. Results: Abnormal haemoglobin fractions on HPLC were seen in 50 cases. Of this beta-thalassemia was the predominant. There were 16 (32%) cases of beta-thalassemia major and 34 (68%) cases of beta-thalassemia carriers possessing HbA1c(0.16), HbF(0.72),HbA0(0.5), HbE(0.44), HbD(0.36), HbS(0.5), HbA2(0.46), and HbC(0.42). Automated HPLC and beta thalassemia program is an appropriate approach for the screening and presumptive identification of patients as well as carrier of beta-thalassemia prior to DNA studies for definitive diagnosis.